文章基本信息

标题：Bar-Coded Enterobacteria: An Undergraduate Microbial Ecology Laboratory Module
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作者：Thao Le ; James Trexel ; Alexandra Brugler 等
期刊名称：American Journal of Educational Research
印刷版ISSN：2327-6126
电子版ISSN：2327-6150
出版年度：2013
卷号：1
期号：1
页码：26-30
DOI：10.12691/education-1-1-6
语种：English
出版社：Science and Education Publishing
摘要：Microbial community ecology is an area of rapid growth centered within the larger discipline of microbiology. Newly developed research methods using molecular strategies have transformed this area into an accessible research topic. Despite such advances, transmission of this topic into pedagogical form has lagged behind. To improve this situation, an undergraduate research team created an artificial microbial community for class room use. They used color-coded enterobacterial taxa transformed with broad-host range plasmids that encoded green fluorescent protein color variants. Using this instructional tool, a class room teaching module was developed about microbial fitness. Over a multi-semester period, the module was introduced into a conventional microbiology curriculum and refined. The learning outcomes for this module include; understanding community composition, that the members of a community can respond in different ways to external events and, that these responses can be used to measure fitness. Learning outcomes were measured through pre and post testing and indicated a gain in understanding about microbial communities.
关键词：Microbial Ecology; Laboratory Exercise; Enterobacteria; Bar-coding; GFP