文章基本信息

标题：Application of PCR-RFLP method to determine polymorphism in BMP-15 and GDF9 fecundity genes in Northeast Bulgarian Merino sheep breed
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作者：Milena Bozhilova-Sakova ; Milena Bozhilova-Sakova ; Ivona Dimitrova 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2021
卷号：10
期号：2
页码：107-111
语种：English
出版社：Plovdiv University Press
摘要：The purpose of this study was to determine the presence or absence of mutations in BMP-15 and GDF9 fecundity genes. In this experiment 60 ewes from Northeast Bulgarian Merino sheep breed were tested for polymorphisms in BMP-15 gene and SNP G1 of the GDF9 gene. This is the first investigation of BMP-15 gene by PCR-RFLP method in Bulgarian sheep breed. After DNA extraction and PCR amplification were received the expected PCR products. The digestion with restriction enzyme HinfI for BMP-15 gene and HhaI for GDF0 revealed mutations in both genes. In BMP-15 were found alleles + and G with frequencies 0.94 and 0.06, respectively and genotypes ++ and G+ with frequencies 0.80 and 0.12, respectively. In GDF9 were identified alleles G and A with frequencies 0.94 and 0.06 and genotypes GG and AG with frequencies 0.88 and 0.12.