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标题：A recombinant VSV-vectored vaccine rapidly protects nonhuman primates against lethal Nipah virus disease
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作者：Stephanie L. Foster ; Courtney Woolsey ; Viktoriya Borisevich 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2022
卷号：119
期号：12
DOI：10.1073/pnas.2200065119
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Significance Concern has increased about the pandemic potential of Nipah virus (NiV). Similar to SARS-CoV-2, NiV is an RNA virus that is transmitted by respiratory droplets. There are currently no NiV vaccines licensed for human use. While several preventive vaccines have shown promise in protecting animals against lethal NiV disease, most studies have assessed protection 1 mo after vaccination. However, in order to contain and control outbreaks, vaccines that can rapidly confer protection in days rather than months are needed. Here, we show that a recombinant vesicular stomatitis virus vector expressing the NiV glycoprotein can completely protect monkeys vaccinated 7 d prior to NiV exposure and 67% of animals vaccinated 3 d before NiV challenge. Nipah virus (NiV) is an emerging highly lethal zoonotic disease that, like SARS-CoV-2, can be transmitted via respiratory droplets. Single-injection vaccines that rapidly control NiV outbreaks are needed. To assess the ability of a vaccine to induce fast-acting protection, we immunized African green monkeys with a recombinant vesicular stomatitis virus (VSV) expressing the Bangladesh strain glycoprotein (NiV BG) of NiV (rVSV-ΔG-NiV BG). Monkeys were challenged 3 or 7 d later with a lethal dose of NiV B. All monkeys vaccinated with rVSV-ΔG-NiV BG 7 d prior to NiV B exposure were protected from lethal disease, while 67% of animals vaccinated 3 d before NiV B challenge survived. Vaccine protection correlated with natural killer cell and cytotoxic T cell transcriptional signatures, whereas lethality was linked to sustained interferon signaling. NiV G-specific antibodies in vaccinated survivors corroborated additional transcriptomic findings, supporting activation of humoral immunity. This study demonstrates that rVSV-based vaccines may have utility in rapidly protecting humans against NiV infection.
关键词：enNipah virushenipavirusvaccinerVSV-NiVrecombinant vesicular stomatitis virus