文章基本信息

标题：No substantial preexisting B cell immunity against SARS-CoV-2 in healthy adults
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作者：Meryem Seda Ercanoglu ; Lutz Gieselmann ; Sabrina Dähling 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：3
页码：1-20
DOI：10.1016/j.isci.2022.103951
语种：English
出版社：Elsevier
摘要：SummaryPreexisting immunity against SARS-CoV-2 may have critical implications for our understanding of COVID-19 susceptibility and severity. The presence and clinical relevance of a preexisting B cell immunity remain to be fully elucidated. Here, we provide a detailed analysis of the B cell immunity to SARS-CoV-2 in unexposed individuals. To this end, we extensively investigated SARS-CoV-2 humoral immunity in 150 adults sampled pre-pandemically. Comprehensive screening of donor plasma and purified IgG samples for binding and neutralization in various functional assays revealed no substantial activity against SARS-CoV-2 but broad reactivity to endemic betacoronaviruses. Moreover, we analyzed antibody sequences of 8,174 putatively SARS-CoV-2-reactive B cells at a single cell level and generated and tested 158 monoclonal antibodies. None of these antibodies displayed relevant binding or neutralizing activity against SARS-CoV-2. Taken together, our results show no evidence of competent preexisting antibody and B cell immunity against SARS-CoV-2 in unexposed adults.Graphical abstractDisplay OmittedHighlights•Comprehensive analysis of the B cell response to SARS-CoV-2 in pre-pandemic samples•No substantial plasma and IgG reactivity against SARS-CoV-2•MAbs isolated from pre-pandemic samples showed no SARS-CoV-2 neutralizing activity•No indication of competent preexisting B cell immunity against SARS-CoV-2Immunity; Immune response; Virology