文章基本信息

标题：Mining RNAseq data reveals dynamic metaboloepigenetic profiles in human, mouse and bovine pre-implantation embryos
本地全文：下载
作者：Marcella Pecora Milazzotto ; Michael James Noonan ; Marcia de Almeida Monteiro Melo Ferraz 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：3
页码：1-23
DOI：10.1016/j.isci.2022.103904
语种：English
出版社：Elsevier
摘要：SummaryMetaboloepigenetic regulation has been reported in stem cells, germ cells, and tumor cells. Embryonic metaboloepigenetics, however, have just begun to be described. Here we analyzed RNAseq data to characterize the metaboloepigenetic profiles of human, mouse, and bovine pre-implantation embryos. In embryos, metaboloepigenetic reprogramming was species-specific, varied with the developmental stage and was disrupted within vitroculture. Metabolic pathways and gene expressions were strongly correlated with early embryo DNA methylation and were changed within vitroculture. Although the idea that thein vitroenvironment may influence development is not new, there has been little progress on improving pregnancy rates after decades usingin vitrofertilization. Hence, the present data will contribute to understanding how thein vitromanipulation affects the metaboloepigenetic status of early embryos, which can be used to establish culture strategies aimed at improving thein vitroenvironment and, consequently, pregnancy rates and offspring health.Graphical abstractDisplay OmittedHighlights•Embryonic metaboloepigenetic reprogramming is stage- and species-specific•In vitroculture disrupts thein vivoembryonic metaboloepigenetic reprogramming•Metabolic genes and pathways are highly correlated with embryo methylomeBiological sciences; Molecular biology; Endocrinology; Omics; Transcriptomics