文章基本信息

标题：Methane Mitigation and Microbial Diversity of Silage Diets Containing Calliandra calothyrsus in a Rumen in Vitro Fermentation System
本地全文：下载
作者：R Ridwan ; I Rusmana ; Y Widyastuti 等
期刊名称：Tropical Animal Science Journal
印刷版ISSN：2615-787X
电子版ISSN：2615-790X
出版年度：2014
卷号：37
期号：2
页码：121-128
DOI：10.5398/medpet.2014.37.2.121
语种：English
出版社：Bogor Agricultural University
摘要：This study was conducted to investigate the effects of silage based diets on methane (CH4) mitigation and microbial diversity in a rumen in vitro fermentation. The experiment was arranged in a completely randomized design with five treatments and three replications. The dietary treatments consisted of varying levels of silage containing 50% Calliandra calothyrsus as follows K; 100% concentrate + pure tannic acid of 1 mg/mL, R1; 25% silage + 75% concentrate, R2; 50% silage + 50% concentrate, R3; 75% silage + 25% concentrate, and R4; 100% silage. The fermentation variables measured were total gas, CH4, in vitro organic matter digestibility (IVOMD), VFAs, pH, N-NH3, number of protozoa, and microbial diversity analysis. Increasing level of silages reduced total gas production, CH4 concentration, IVOMD, index of bacterial diversity, protozoal number, total methanogens and Methanobacteriales population. Diet with 25% to 50% silage decreased CH4 concentration, total gas production and IVOMD by 11.43%, 24.92%, and 18.73%, respectively. Ammonia N and VFAs (except butyrate and valerate) were significantly reduced (P<0.01) by increasing level of silages in the ration. In conclusion, this study confirmed that 50% silage containing C. calothyrsus was efficient in mitigation of enteric CH4 production by reducing total methanogens and Methanobacteriales number, but had negative effect on decreasing bacterial diversity and organic matter digestibility.
其他摘要：Penelitian ini dilakukan untuk mengevaluasi pengaruh pakan silase yang mengandung 50% Calliandra calothyrsus pada mitigasi metan (CH4 ) dan keragaman mikroba dalam fermentasi rumen secara in vitro. Penelitian ini menggunakan rancangan acak lengkap dengan 5 perlakuan dan 3 ulangan. Perlakuan terdiri atas K; 100% konsentrat + asam tanat murni (1 mg/ml), R1; 25% silase + 75% konsentrat, R2; 50% silase + 50% konsentrat, R3; 75% silase + 25% konsentrat, dan R4; 100% silase. Variabel fermentasi yang diukur terdiri atas total gas, konsentrasi CH4 , kecernaan in vitro bahan organik (IVOMD), VFAs, pH, N-NH3 , jumlah protozoa, dan analisis keragaman mikroba rumen. Hasil penelitian menunjukkan peningkatan level silase menurunkan produksi gas total, konsentrasi CH4 , IVOMD, indeks keragaman bakteri, jumlah protozoa, populasi metanogen dan Methanobacterales. Penggunaan silase 25%-50% menurunkan CH4 , gas total dan IVOMD secara beurutan sebesar 11,43%, 24,92%, dan 18,73%. Nitrogen ammonia dan VFAs (kecuali butirat and valerat) secara nyata (P<0,01) mengalami penurunan dengan meningkatnya level silase yang digunakan. Penelitian ini menegaskan bahwa penggunaan 50% pakan silase yang mengandung C. calothyrsus efisien dalam mitigasi enterik CH4 dengan menurunkan jumlah metanogen dan Methanobacteriales, tetapi masih memiliki pengaruh negatif terhadap penurunan keragaman bakteri dan kecernaan bahan organik.
关键词：Calliandra calotyhrsus;silage;rumen fermentation;methane;microbial diversity
其他关键词：Calliandra calothyrsus;silase;fermentasi rumen;metan;keragaman mikroba