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标题：Profiles of Cortisol, Triiodothyronine, Thyroxine and Neutrophil/Lymphocyte Ratio as Stress Indicators in Swamp Buffaloes 15 Days Post-Transportation
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作者：H Maheshwari ; . Yulnawati ; A Esfandiari 等
期刊名称：Tropical Animal Science Journal
印刷版ISSN：2615-787X
电子版ISSN：2615-790X
出版年度：2013
卷号：36
期号：2
页码：106-112
DOI：10.5398/medpet.2013.36.2.106
语种：English
出版社：Bogor Agricultural University
摘要：Transportation may cause stress that affects livestock’s health. This research was conducted to observe the effect of transportation on the profiles of blood cortisol, triiodothyronine (T3), thyroxine (T4) and neutrophil/lymphocyte (N/L) ratio as stress indicators during 15 days post-transportation. Four females swamp buffaloes, 2-yr-old were used in this research. The animals were transported using an open truck, along 15 kilometers distance for 2 h. During acclimatization, the animals were fed grass twice a day and access to water ad libitum. Blood was collected at the time of arrival and everyday for 15 d post-transportation. Cortisol, T3 and T4were analyzed using Radioimmunoassay (RIA) method. Blood smears were also prepared and stained with Giemsa for leukocyte differential counts. Results showed a significant relation (P<0.05) between transport and an increase in cortisol and T4 level on day-1 post-transportation compared to the normal levels. The level of T3 was also increased on day-3 post-transportation. Total leukocyte counts were 8.91-18.83×10³/μL. Neutrophil, eosinophil, monocyte, and lymphocyte counts were 4.87-10.41×10³/μL, 0.66-1.75×10³/μL, 0.11-0.58×10³/μL, and 2.98-6.21×10³/μL, respectively. N/L ratio was 1.23-3.49 with >1.5 in average throughout the period of the research. It is concluded that transportation causes stress and therefore changes metabolic process.
其他摘要：Transportasi dapat menyebabkan suatu kondisi stres yang berdampak terhadap kesehatan ternak. Penelitian ini bertujuan untuk mengkaji pengaruh transportasi terhadap kadar kortisol, triiodotironin (T3), tiroksin (T4) darah dan rasio neutrofil/limfosit (N/L) sebagai indikator cekaman selama 15 hari pasca-transportasi. Empat ekor kerbau lumpur betina usia 2 tahun digunakan dalam penelitian ini. Kerbau-kerbau tersebut ditranspor menggunakan truk bak terbuka menempuh jarak 15 km selama 2 jam. Selama 15 hari masa aklimatisasi, kerbau-kerbau tersebut diberi pakan rumput 2 kali sehari dan air minum ad libitum. Pengambilan darah dilakukan setiap pagi hari sejak tiba di lokasi selama 15 hari pasca-transportasi. Kadar kortisol, T3, dan T4 diukur menggunakan metode radio-immunoassay (RIA). Diferensiasi leukosit dilakukan menggunakan preparat ulas darah yang telah diwarnai dengan pewarna Giemsa. Hasil yang diperoleh menunjukkan bahwa terdapat korelasi yang signifikan (P<0,05) antara transportasi dan peningkatan kadar kortisol dan T4 pada hari ke-1 pasca-transportasi bila dibandingkan dengan kadar normal, sedangkan peningkatan kadar T3 terlihat pada hari ke-3pasca-transportasi. Kisaran jumlah leukosit total adalah 8,91-18,.83×10³/μl, jumlah neutrofil, eosinofil, monosit, dan limfosit secara berturut-turut adalah 4,87-10,41×10³/μl, 0,66-1,75×10³/μl, 0,11-0,58×10³/μl, dan 2,98-6,21×10³/μl. Rasio N/L berkisar antara 1,23-3,49 dengan rerata >1,5. Dari hasil yang diperoleh dapat disimpulkan bahwa hewan mengalami cekaman transportasi sehingga proses metabolisme mengalami perubahan.
关键词：cortisol;N/L ratio;swamp buffalo;thyroxine;triiodothyronine
其他关键词：kortisol;rasio N/L;kerbau lumpur;tiroksin;triiodotironin