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标题：The Characteristics of Cytochrome C Oxidase Gene Subunit I in Wild Silkmoth Cricula trifenestrata Helfer and Its Evaluation for Species Marker
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作者：. Suriana ; D D Solihin ; R R Noor 等
期刊名称：Tropical Animal Science Journal
印刷版ISSN：2615-787X
电子版ISSN：2615-790X
出版年度：2012
卷号：35
期号：2
页码：102-110
DOI：10.5398/medpet.2012.35.2.102
语种：English
出版社：Bogor Agricultural University
摘要：The study was conducted to assess the characteristics of partial gene of cytochrome C oxidase subunit I (COI) of wild silkmoth Cricula trifenestrata, and to detect the diagnostic sites from these gene for evaluation as species marker. A total of fifteen larvae of C. tifenestrata were collected from Bogor, Purwakarta, and Bantul Regencies. Genomic DNA was extracted from silk gland of individual larvae, then amplified by PCR method and sequenced. DNA sequencing was done to characterize their nucleotide and amino acid contents. The results showed that 595 nucleotides at the 5 'end of COI gene of C. tifenestrata was conserved at the species level, but varies at the family level. Nucleotide dominated by thymine and adenine bases (± 70%). There were 25 diagnostic sites for C. tifenestrata, and four diagnostic sites for genus level. One hundred eigthty nine (189) amino acids were alignment, and only one percent of the genes was varied among species. The 107th amino acid (valine) and 138th(threonine) were diagnostics amino acid for C. tifenestrata. Based on nucleotides and amino acids sequences, the phylogeny showed that C. tifenestrata lied on the same nodes with Antheraea, so the Saturniidae family is monophyletic.
其他摘要：Penelitian bertujuan untuk mengkarakterisasi dan mendeteksi situs diagnostik dari parsial gen sitokrom C oksidase sub unit I (COI) ulat sutera liar Cricula trifenestrata, dan mengevaluasi gen tersebut sebagai penanda spesies. Sebanyak 15 larva C. tifenestrata dikoleksi dari Kabupaten Bogor, Purwakarta, dan Bantul. DNA genom diekstrak dari kelenjar sutera larva, kemudian diperbanyak dengan metode PCR dan disekuensi. Hasil sekuensi dikarakterisasi runutan nukleotida dan diprediksi kandungan asam aminonya. Hasilnya menunjukkan bahwa 595 runutan nukleotida pada ujung 5’ gen COI C. tifenestrata bersifat kekal pada level spesies, tetapi beragam pada level family. Nukleotida didominasi oleh basa timin dan adenin (± 70%). Terdapat 25 situs diagnostik bagi C. tifenestrata dan 4 situk diagnostik pada level genus. Seratus delapan puluh sembilan (189) asam amino yang disejajarkan, menunjukkan satu persen variasi antar spesies. Asam amino ke-107 (valin) dan asam amino ke-138 (treonin) merupakan asam amino diagnostik bagi C. tifenestrata. Berdasarkan runutan nukleotida dan asam aminonya, filogeni kekerabatan menunjukkan bahwa C. tifenestrata terdapat pada nodus yang sama dengan spesies Antheraea, sehingga family Saturniidae bersifat monofiletik.
关键词：wild silk moth;C. trifenestrata;cytohrome C oxidase subunit I;nucleotide diagnostic;amino acid diagnostic
其他关键词：ulat sutera liar;C. trifenestrata;sitokrom oksidase sub unit I;nukleotida diagnostik;asam amino diagnostik