文章基本信息

标题：Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows
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作者：R Misrianti ; C Sumantri ; A Farajallah 等
期刊名称：Tropical Animal Science Journal
印刷版ISSN：2615-787X
电子版ISSN：2615-790X
出版年度：2010
卷号：33
期号：3
页码：131-136
DOI：10.5398/medpet.2010.33.3.131
语种：English
出版社：Bogor Agricultural University
摘要：Pit-1 gene has been identified as the pituitary specific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total of 320 Indonesian buffaloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH cattle. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buffaloes.
关键词：buffal;Pit1 gene;PCR RFLP