文章基本信息

标题：Strategies for Improving Small-Molecule Biosensors in Bacteria
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作者：Corwin A. Miller ; Joanne M. L. Ho ; Matthew R. Bennett 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：2
DOI：10.3390/bios12020064
语种：English
出版社：MDPI Publishing
摘要：In recent years, small-molecule biosensors have become increasingly important in synthetic biology and biochemistry, with numerous new applications continuing to be developed throughout the field. For many biosensors, however, their utility is hindered by poor functionality. Here, we review the known types of mechanisms of biosensors within bacterial cells, and the types of approaches for optimizing different biosensor functional parameters. Discussed approaches for improving biosensor functionality include methods of directly engineering biosensor genes, considerations for choosing genetic reporters, approaches for tuning gene expression, and strategies for incorporating additional genetic modules.
关键词：enwhole-cell biosensorbacterial biosensorbiosensor engineeringprotein engineeringgenetic engineeringgenetic circuits