文章基本信息

标题：How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application
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作者：Escarlet Díaz-Galicia ; Raik Grünberg ; Stefan T. Arold 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：2
DOI：10.3390/bios12020053
语种：English
出版社：MDPI Publishing
摘要：CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a given application among thousands of potential candidates. We provide an overview of the currently described Cas effector systems and review existing Cas-based RNA detection methods. We then propose a set of systematic selection criteria for selecting CRISPR-Cas candidates for new applications. Using this approach, we identify four candidates for in vitro RNA.
关键词：enRNA sensingCRISPR-Casspacerin vitroPAMsensor design