文章基本信息

标题：Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application
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作者：Fifame Auriane Oussou-Azo ; Taiki Futagami ; Mun’delanji Catherine M. Vestergaard 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：2
DOI：10.3390/bios12020049
语种：English
出版社：MDPI Publishing
摘要：Early and quick detection of pathogens are crucial for managing the spread of infections in the biomedical, biosafety, food, and agricultural fields. While molecular diagnostics can offer the specificity and reliability in acute infectious diseases, detection of pathogens is often slowed down by the current benchtop molecular diagnoses, which are time consuming, labor intensive, and lack the mobility for application at the point-of-need. In this work, we developed a complete on-farm use detection protocol for the plant-devastating anthracnose agent: Colletotrichum gloeosporioides. Our methods combined a simplified DNA extraction on paper that is compatible with loop-mediated isothermal amplification (LAMP), coupled with paper-based immunoassay lateral flow sensing. Our results offer simple, quick, easy, and a minimally instrumented toolkit for Colletotrichum gloeosporioides detection. This scalable and adaptable platform is a valuable alternative to traditional sensing systems towards on-the-go pathogen detection in food and agriculture, biomedical, and other fields.
关键词：enColletotrichum gloeosporioidesanthracnoseimmuno-dipstickloop-mediated amplification (LAMP)simplified DNA extractionpoint-of-need molecular diagnostic