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标题：The Composition and Anti-Aging Activities of Polyphenol Extract from Phyllanthus emblica L. Fruit
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作者：Min Wu ; Jianhang Cai ; Zhengfeng Fang 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2022
卷号：14
期号：4
DOI：10.3390/nu14040857
语种：English
出版社：MDPI Publishing
摘要：Phyllanthus emblica L. (PE) is commonly known as a medicine and food homologous plant, which is abundant in natural products polyphenols. In the present study, polyphenols were extracted from PE fruit by response surface method, and the anti-aging ability was determined. PE fruit polyphenols exhibited strong antioxidant capacities in scavenging free radicals, and anti-cholinesterase ability by inhibition of AChE (IC 50 0.2186 ± 0.0416 mg/mL) and BuChE (IC 50 0.0542 ± 0.0054 mg/mL) in vitro. Moreover, PE fruit polyphenols showed strong protective effect against the aging process in Caenorhabditis elegans model, including increased thermal resistance, extended lifespan by 18.53% ( p < 0.05), reduced activity of AChE by 34.71% and BuChE by 45.38% ( p < 0.01). This was accompanied by the enhancement in antioxidant enzymes activity of SOD by 30.74% ( p < 0.05) and CAT by 8.42% ( p > 0.05), while decrease in MDA level by 36.25% ( p < 0.05). These properties might be interrelated with the presence of abundant flavonols and phenolic acids identified by UPLC-ESI-QTOF-MS, such as quercetin, myricetin, ellagic, gallic, and chlorogenic acids, together with their glycosides. The remarkable antioxidant and anti-aging potential of PE fruit polyphenols could be implemented in the food and pharmaceutical industry.
关键词：enmedicine and food homologous plant substancesnatural productsbioactivity evaluationpolyphenolsantioxidantanti-aging