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标题：Detection of pathogens of veterinary importance harboured by Stomoxys calcitrans in South African feedlots
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作者：Nokofa B. Makhahlela ; Danica Liebenberg ; Huib Van Hamburg 等
期刊名称：Scientific African
印刷版ISSN：2468-2276
出版年度：2022
卷号：15
页码：1-7
DOI：10.1016/j.sciaf.2022.e01112
语种：English
出版社：Elsevier
摘要：AbstractStable flies are blood feeding ectoparasites of economic, veterinary and medical importance. There is scarcity of data on pathogens harboured by stable flies occurring in South African feedlots. Hence, in this study we documented stable flies occurring in three selected South African feedlots and carried out a PCR based detection for the presence of DNA ofA. marginale, Lumpy skin disease virus (LSDV),Ehrlichiaspp. andRickettsiaspp. The flies were captured with vavoua traps, identified morphologically asStomoxys calcitranswhich was also supported by amplification and sequencing of both16S rRNA and COI genes which matched with relevant species on the NCBI database. A total of 53 fly pools were used for the detection of pathogen DNA. PCR showed the presence of DNA ofAnaplasma marginale(22.64%, 12/53) and LSDV (15.08%; 8/53). NeitherRickettsianorEhrlichiaspp. DNA were detected from all the sampled feedlots. In conclusion, results obtained from this study showed thatS. calcitransin feedlots is harbouringA. marginaleand LSDV which suggests that they may be involved in their mechanical transmission to livestock.
关键词：KeywordsStable flyStomoxys calcitransAnaplasma marginaleRickettsiaspp.Lumpy skin disease virus