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标题：Association of neutrophil extracellular traps with the production of circulating DNA in patients with colorectal cancer
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作者：Brice Pastor ; Jean-Daniel Abraham ; Ekaterina Pisareva 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：2
页码：1-19
DOI：10.1016/j.isci.2022.103826
语种：English
出版社：Elsevier
摘要：SummaryWe postulate that a significant part of circulating DNA (cirDNA) originates in the degradation of neutrophil extracellular traps (NETs). In this study, we examined the plasma level of two markers of NETs (myeloperoxidase (MPO) and neutrophil elastase (NE)), as well as cirDNA levels in 219 patients with a metastatic colorectal cancer (mCRC), and in 114 healthy individuals (HI). We found that in patients with mCRC the content of these analytes was (i) highly correlated, and (ii) all statistically different (p < 0.0001) than in HI (N = 114). These three NETs markers may readily distinguish between patients with mCRC from HI, (0.88, 0.86, 0.84, and 0.95 AUC values for NE, MPO, cirDNA, and NE + MPO + cirDNA, respectively). Concomitant analysis of anti-phospholipid (anti-cardiolipin), NE, MPO, and cirDNA plasma concentrations in patients with mCRC might have value for thrombosis prevention, and suggested that NETosis may be a critical factor in the immunological response/phenomena linked to tumor progression.Graphical abstractDisplay OmittedHighlights•NETs markers correlate with cirDNA amounts in patients with mCRC not in healthy subjects•Quantifying NETs markers and cirDNA could distinguish mCRC from healthy subjects•Analysis of NETs markers, cirDNA, and aPL may have value for thrombosis prevention•A strong fraction of cirDNA concentration could be derived from NETs in patients with mCRCMolecular biology; Immunology