文章基本信息

标题：Obesity modulates cell-cell interactions during ovarian folliculogenesis
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作者：Xin Long ; Qiuyun Yang ; Jingjing Qian 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：1
页码：1-26
DOI：10.1016/j.isci.2021.103627
语种：English
出版社：Elsevier
摘要：SummaryObesity is known to affect female reproduction, as evidenced by obese patients suffering from subfertility and abnormal oogenesis. However, the underlying mechanisms by which obesity impairs folliculogenesis are poorly documented. Here, we performed comprehensive single-cell transcriptome analysis in both regular diet (RD) and obese mouse models to systematically uncover how obesity affects ovarian follicle cells and their interactions. We found an increased proportion ofInhbbhighly expressed granulosa cells (GCs) among all the GC subpopulations in obese mice. Under obese conditions, excessive androgen secreted from endocrine theca cells (ETCs) may contribute to the imbalanced change of GC subtypes through ETCs-GCs interactions. This is alleviated by enzalutamide, an androgen receptor antagonist. We also identified and confirmed typical GC markers, such as Marcks and Prkar2b, for sensitive evaluation of female fertility in obesity. These data represent a resource for studying transcriptional networks and cell-cell interactions during folliculogenesis under physiological and pathological conditions.Graphical abstractDisplay OmittedHighlights•Single-cell transcriptome of folliculogenesis in regular diet-fed and obese mice•ETCs represent higher activity in androgen synthesis under obese conditions•Obesity contributes to the disproportion of GC subtypes under excessive androgen•Enzalutamide alleviates defects in granulosa cells from obese miceBiological sciences; Endocrinology; Cell biology; Transcriptomics