文章基本信息

标题：Standardized two-step testing of antibody activity in COVID-19 convalescent plasma
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作者：Pavlo Gilchuk ; Isaac Thomsen ; Sandra Yoder 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：1
页码：1-22
DOI：10.1016/j.isci.2021.103602
语种：English
出版社：Elsevier
摘要：SummaryThe COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.Graphical abstractDisplay OmittedHighlights•A high-throughput approach enabling antibody activity testing in COVID-19 plasma•SARS-CoV-2 IgG binding screen coupled with quantitative neutralization testing•Neutralization testing is necessary for profiling COVID-19 convalescent plasma•Two broad monoclonal antibodies identified as a neutralization testing standardImmunology; Virology