文章基本信息

标题：Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge
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作者：Nan Xiang ; Zhonghua Ni
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：1
DOI：10.3390/bios12010014
语种：English
出版社：MDPI Publishing
摘要：Conventional sample preparation techniques require bulky and expensive instruments and are not compatible with next-generation point-of-care diagnostic testing. Here, we report a manually operated syringe-tip inertial microfluidic centrifuge (named i-centrifuge) for high-flow-rate (up to 16 mL/min) cell concentration and experimentally demonstrate its working mechanism and performance. Low-cost polymer films and double-sided tape were used through a rapid nonclean-room process of laser cutting and lamination bonding to construct the key components of the i-centrifuge, which consists of a syringe-tip flow stabilizer and a four-channel paralleled inertial microfluidic concentrator. The unstable liquid flow generated by the manual syringe was regulated and stabilized with the flow stabilizer to power inertial focusing in a four-channel paralleled concentrator. Finally, we successfully used our i-centrifuge for manually operated cell concentration. This i-centrifuge offers the advantages of low device cost, simple hand-powered operation, high-flow-rate processing, and portable device volume. Therefore, it holds potential as a low-cost, portable sample preparation tool for point-of-care diagnostic testing.
关键词：eninertial microfluidicscell concentrationhand-poweredpoint-of-care diagnostic testing