文章基本信息

标题：Comprehensive analysis of N6-methyladenosine (m ⁶A) modification during the degeneration of lumbar intervertebral disc in mice
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作者：Bin Zhu ; Hao-xiang Chen ; Shan Li 等
期刊名称：Journal of Orthopaedic Translation
印刷版ISSN：2214-031X
出版年度：2021
卷号：31
页码：126-138
DOI：10.1016/j.jot.2021.10.008
语种：English
出版社：Elsevier B.V.
摘要：Objective To study the N6-methyladenosine (m 6A) modification pattern of nucleus pulposus (NP) tissue during intervertebral disc degeneration (IDD). Methods A standing mouse model was generated, and staining and imaging methods were used to evaluate the IDD model. Methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) was used to analyze m 6A methylation-associated transcripts in the NP, and real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of methylation-related enzymes and conduct bio-informatics analysis. Results The standing mouse model caused IDD. Continuous axial pressure changed the expression of related methylases in degenerated NP tissue. Relative to the control group, the expression levels of KIAA1429, METTL14, METTL3, METTL4, WTAP, DGCR8, EIF3A and YTHDC1 in the experimental group were higher, while those of FTO, ELAVL1, HNRNPC1 and SRSF2 were lower. We identified 985 differentially expressed genes through MeRIP-Seq, among which 363 genes were significantly up-regulated, and 622 genes were significantly down-regulated. In addition, among the 9648 genes counted, 1319 m 6A peaks with significant differences in methylation were identified, among which 933 were significantly up-regulated, and 386 were significantly down-regulated. Genes and pathways that were enriched in IDD have been identified. Conclusion The results of this study elucidated the m 6A methylation pattern of NP tissue in degenerated lumbar intervertebral disc of mice and provided new perspectives and clues for research on and the treatment of lumbar disc degeneration. The Translational potential of this article As one of the important causes of low back and leg pain, intervertebral disc degeneration brings a huge economic burden to the society, family and medical system. Therefore, understanding the molecular and cellular mechanisms of intervertebral disc degeneration is of great significance for guiding clinical treatment. In this study, methylated RNA immunoprecipitation with next-generation sequencing on mice lumbar nucleus pulposus tissues found that differentially expressed genes and changes in the expression of related methylases, confirming that RNA methylation is involved in intervertebral disc degeneration. The process provides new vision and clues for future research on intervertebral disc degeneration.
关键词：Intervertebral disc degeneration; nucleus pulposus; m6A methylation; Methylated RNA immunoprecipitation with next-generation sequencing