文章基本信息

标题：Inhibitory Effect of Astaxanthin on Gene Expression Changes in Helicobacter pylori-Infected Human Gastric Epithelial Cells
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作者：Suhn Hyung Kim ; Hyeyoung Kim ; M. Luisa Bonet 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2021
卷号：13
期号：12
DOI：10.3390/nu13124281
语种：English
出版社：MDPI Publishing
摘要：Helicobacter pylori ( H. pylori) infection promotes gastric carcinogenesis by increasing oxidative stress, inflammation, and dysregulation of cell survival and proliferation of gastric epithelial cells. Astaxanthin (ASTX), a bioactive carotenoid, exhibits antioxidant and anticancer effects by modulating aberrant signaling pathways that lead to dysregulation of cell death and proliferation. To elucidate the molecular mechanism of H. pylori-induced gastric carcinogenesis and to examine the inhibitory effect of ASTX on H. pylori-induced gastric epithelial cell gene expression changes, we performed comparative RNA-sequencing (RNA-Seq) analysis for H. pylori-infected gastric epithelial cells treated with or without ASTX. RNA-Seq results reveal that differentially expressed genes (DEGs) in H. pylori-infected cells were mainly associated with the Wnt/β-catenin signaling pathway, which is related to cell proliferation. ASTX significantly reversed H. pylori-induced transcriptional alterations of the key mediators involved in β-catenin signaling, notably, porcupine (gene symbol, PORCN), spermine oxidase ( SMOX), bone morphogenetic protein (BMP) and activin membrane-bound inhibitor ( BAMBI), SMAD family member 4 ( SMAD4), transforming growth factor-β1 ( TGFB1), Fos-like 1 ( FOSLI), and c-myc ( MYC). We suggest that ASTX may be a potential therapeutic agent that can suppress H. pylori-induced proliferation-associated gene expression changes, in part, by counter-regulating the Wnt/β-catenin signaling pathway.
关键词：Helicobacter pylori; gastric epithelial cells; astaxanthin; β-catenin; cell proliferation; gastric cancer