文章基本信息

标题：ASSESSMENT OF ANTI-PROLIFERATIVE AND APOPTOTIC ACTIVITIES OF Centaurothamnus maximus AND GC-MS ANALYSIS OF BIOACTIVE FRACTION
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作者：Fahd A Nasr ; Ali S Alqahtani ; Amal A Alotaibi 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2021
卷号：30
期号：8
页码：10297-10305
语种：English
出版社：PSP Publishing
摘要：Cancer continues to be a major health problem globally. Historically, plants have been studied for their anticancer effects. Centaurothamnus maximus is a native plant species found in Saudi Arabia and Yemen and is one of the least studied plants for its chemical constituents or biological activities. The present study aimed to explore the cytotoxic poten-tial of C. maximus using different cell lines and con-duct phytochemical analysis utilizing GC-MS anal-ysis. Further, analysis of cell cycle and apoptosis in-duction were also conducted using flow cytometry. In this study, we found that C. maximus chloroform fraction(CMCF)exhibited the highest cytotoxicity towards MCF-7 cells with an ICs value of 25.3 ug/mL. Follow cytometric analysis displayed that CMCF led to dose-dependent accumulation of cells in G1 phase and increase the number of apoptotic cells as shown by propidium iodide and Annexin V-FITC/PI staining respectively. Tritetracontane (27.14 %), lupeol(19.87 %), E-15-heptadecenal (10.4%)and phytol (3,7,11,15-tetramethyl-2-hex-adecen-1-ol)(8.35%)were the major constituents in CMCF.Overall,this study represents the first find-ings showing that C. maximus trigger antiprolifera-tion activity via cell cycle arrest and apoptotic induc-tion. The results of the present study confirmed the cytotoxic activity of C. maximus and further investi-gations can be done to understand its molecular mechanisms.
关键词：C maximus;Cell cycle;Apoptosis;GC-MS