文章基本信息

标题：IDENTIFICATION OF APHID SPECIES ISOLATED FROM TOMATO CROPS USING PHYLOGENETIC APPROACHES IN RIYADH AND HAFAR AL-BATIN,SAUDI ARABIA
本地全文：下载
作者：Dina M Metwally ; Reem A Alajmi ; Mona DAl-Shammari 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2021
卷号：30
期号：8
页码：9763-9770
语种：English
出版社：PSP Publishing
摘要：Aphids are common agricultural pests found worldwide and cause significant economic losses to farmers. Although these pests are of economic im-portance,information on their genome is limited. We examined the sequence diversity of two genes, the mitochondrial gene, subunit I of cytochrome oxidase(COI) and the 16S rRNA-encoding se-quence of aphids sourced from tomato farms from two geographical regions of Saudi Arabia (Rivadh and Hafar Al-Batin).Partial sequences of COI (from 12 aphid isolates)and 16S rRNA-encoding sequence(from five aphid isolates) were separately amplified using polymerase chain reaction and se-quenced to reveal the genomic similarity among specimens of 92-99% and 95.7%,respectively.Our data did not indicate any differences between aphid isolates. These results demonstrate that the present species are deeply embedded in the Aphis genus and share a close relationship with other Aphis spe-cies, including Aphis gossypii as a putative sister taxon. These isolates cannot be categorized as be-longing to two different groups with origins in Ri-yadh and Hafar Al-Batin.
关键词：Aphis gossypi;COI;Hafar Al-Batin;16S rRNA;Tomato;Riyadh