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标题：EFFECT OF RHIZOBIUM. NANO SILICA AND ASCORBIC ACID ON MORPHO-PHYSIOLOGICAL CHARACTERS ANDGENE EXPRESSION OF POX AND PPO IN FABA BEAN (VICIA FABA L.) UNDER SALINITY STRESS CONDITIONS
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作者：Rasha F EI-Flaah ; Rania A R El-Said ; Mohammed A Nassar 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2021
卷号：30
期号：6
页码：5751-5764
语种：English
出版社：PSP Publishing
摘要：Faba bean (Vicia faba L.) is one of the greatest important leguminous plants used for human nutri- tion in Egypt, which is the fourth important legumi- nous crop in the world after dry bean, dry peas and chickpea. Salt stress affects all major processes and metabolism in plants, so these experiments were car-ried out in 2018/2019 and 2019/2020 to study the in- fluence of ascorbic acid (200 and 300 mg/L), Rhizo-bium and nano silica (2 and 3 mM) on the physiolog-ical. biochemical parameters and gene expression of peroxidase (POX) as well as polyphenol oxidase (PPO) in faba bean plants under salinity conditions (0, 2000 and 4000 ppm) on Giza 3 cultivar. The re- sults showed that salinity stress adversely affect var- ious morphological and physiological characters in- cluding stem height, number of leaves, number ofbranches, chlorophyll content and relative water content as well as yield components, whereas elec- trolyte leakage increased in salt-stressed plants. The application of ascorbic acid (200 mg/L), Rhizobium and nano silica (2 mM) markedly improved growth parameters, physiological characteristics and yield components of stressed plants compared with un- treated stressed plants. A significant reduction in electrolyte leakage was also recorded for all treat- ments. It was also observed from the results that the gene expression of peroxidase (POX) and polyphe- nol oxidase (PPO) was high in plants treated with nano silica, ascorbic acid and Rhizobium, however, there was a decrease in the gene expression in the stressed untreated plants. Our results revealed the important role of nano silica, ascorbic acid and Rhi- zobium in enhancing faba bean growth and tolerance to salinity stress.
关键词：Vicia faba L;salt stress;ascorbic acid;Rhizobium;nano silica;gene expression