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标题：Method for observing Beauveria bassiana colonization in plants
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作者：Qiu-yang Wei ; Yue-ni Liu
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2021
卷号：8
页码：1-7
DOI：10.1016/j.mex.2021.101364
语种：English
出版社：Elsevier
摘要：AbstractMicrobes interact in a multitude of ways with host plants, can dwell as endophytes within plants causing no apparent disease, and often provide benefits to their host. Observing microorganism distribution and colonization is a prerequisite for interactive research. To this end, we describe use of fluorescent staining for microorganism labeling and highlight its simplicity, and efficiency. Fluorescein can quickly bind toBeauveria bassianaspores, producing bright green fluorescence that can be observed even inside plant tissues. This method provides an intuitive visual image that can be utilised for subsequent data acquisition and statistical analysis.•Our protocol depends on binding of fluorescein diacetate (FDA) specifically to microorganisms. The fungus hydrolyses and metabolises FDA in cells to produce bright green fluorescent products. This fluorescent signal can easily penetrate plant epidermis and be detected by fluorescence microscopy.•FDA, which itself does not emit light, will emit a fluorescent signal only when combined withB. bassiana.Concomitant genetic testing of the fungal ITS confirmed the high level of the fluorescent staining method for detection ofB. bassiana.•Compared with the previous green fluorescent protein (GFP) labeling methods, this protocol improved the labeling efficiency of microorganisms and simplifies the process.Graphical abstractDisplay Omitted
关键词：Fluorescence;Fluorescein diacetate (fda);Solanum lycopersicum;Beauveria bassiana;Endophytes