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标题：ELISA based on peptide antigens reproducing cross-reactive viral epitopes to detect antibodies in latent autoimmune diabetes in adults vs. type 1 diabetes
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作者：Feliciana Real-Fernández ; Alessandra Gallo ; Francesca Nuti 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2021
卷号：8
页码：1-6
DOI：10.1016/j.mex.2021.101452
语种：English
出版社：Elsevier
摘要：AbstractDiagnosis of Latent Autoimmune Diabetes in Adults (LADA) is based on the adult-age, anti-islet autoantibodies, and temporary insulin-independence. As in Type-1-Diabetes (T1DM), autoimmunity may trigger LADA and enteroviruses-infections can play a role. Anti-human Glutamic-Acid-Decarboxylase (hGAD) autoantibodies are accepted clinical biomarkers, but do not discriminate LADAvs.T1DM. The hypothesis is that protein antigens detecting anti-hGAD antibodies do not expose epitopes specific for different disease forms.We investigated the diagnostic value of autoantibodies in LADAvs.T1DM to peptides of hGAD65/67 isoforms, and Enterovirus-Coxsackie-B4 (CVB4), as antigens sharing the epitope PEVKXK (X: E/T) included in CD8 T-cell CVB4 epitope restricted by diabetes-associated HLA-A2.1. Statistically significant differences of IgM and/or IgG in LADA and T1DMvs.controls were identified. In LADA IgMs to GAD65/67 peptides are diagnostics, IgGs to GAD65/67 peptides correlate with anti-CVB4 peptide antibodies. IgM and/or IgG to all tested peptides can predict LADA, monitoring CVB4 infected patients, improving LADAvs.T1DM stratification.•A customized SP-ELISA based on synthetic peptides Ac-hGAD65(250-273)-NH2(1), Ac-hGAD67(258-281)-NH2(2), and Ac-CVB4P2C(28-50)-NH2(3) is described.•The method was designed to detect specific IgM and/or IgG in LADA, T1DM,vs.controls•Final aim is improvement of LADAvs.T1DM patient stratification.Graphical abstractDisplay Omitted
关键词：Glutamic acid decarboxylase;Enterovirus Coxsackie B4;LADA;T1DM