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标题：Genetic detection and phylogenetic relationship of Babesia species infecting domestic dogs from select regions in Kenya
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作者：Ismail Thoya Ngoka ; Kevin Mbogo ; Martina Kyallo 等
期刊名称：Scientific African
印刷版ISSN：2468-2276
出版年度：2021
卷号：14
页码：1-10
DOI：10.1016/j.sciaf.2021.e01010
语种：English
出版社：Elsevier
摘要：AbstractThe genusBabesiahas more than 100 species that are transmitted by ticks with some being zoonotic. They can infect humans, livestock, and wildlife. Although canine babesiosis occurs locally, published studies on the species involved are limited.Babesiaparasites cause severe disease in dogs which can be fatal. Drawbacks of the current control methods necessitate vaccine development.The study objective was to identify theBabesiaspecies infecting dogs from three Kenyan counties; Nairobi, Mombasa, Nakuru and determine their phylogenetic relationship. This will enable improved control and rule out zoonotic potential. The study period was October 2018 to November 2019.The study design was descriptive and sampling opportunistic. One hundred and forty-three dogs were sampled. From whole blood, total DNA was extracted using the TanBead extractor followed by PCR amplification targetingBabesia18S rRNA. Positive samples were purified and sequenced using the Sanger Dideoxy method.CLC Genomics Workbench, GenBank™ and BLASTn™ on NCBI were used for sequence processing and analysis. Geneious prime™ was used for multiple sequence alignment and phylogenetic analysis.The overall prevalence ofBabesia caniswas 9.0% (95% CI: 4.37–13.81). Two out of 13 positive samples (2/13) were identified asBabesia canis vogeli, with a prevalence of 1.4% (95% CI: 1.38 - 14.2,n = 143) while 11/13 were identified asBabesia canis rossi, with a prevalence of 7.69% (95% CI: 3.3 -12,n = 143). TheBabesia rossisequences identified were closely related to sequences from black-backed jackals, while theBabesia vogeliones were related to sequences from a pet cat in China.Babesia rossiwhich causes severe canine babesiosis was identified in 84.6% of the positive samples, immediate and aggressive clinical intervention is necessary. The possible sylvatic cycle ofBabesia rossiand low levels of infections byBabesia vogelishould inform pertinent control measures.
关键词：KeywordsDomestic dogBabesiaparasitesZoonosisWildlifeHuman-livestock-wildlife interaction