文章基本信息

标题：Author Correction: Determinants of duck Tembusu virus NS2A/2B polyprotein procession attenuated viral replication and proliferation in vitro
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作者：Bowen Jiang ; Wei Zhang ; Yuanyuan Wu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-03536-w
语种：English
出版社：Springer Nature
摘要：Correction to: Scientifc Reports 10.1038/s41598-020-68271-0, published online 24 July 2020 The original version of this Article contained an error in Figure 1D where the label indicating “NS2B-Flag(14kDa)” was incorrectly given as “Myc-NS2B(25kDa).” The original Figure 1 and accompanying legend appear below. Figure 1 Cleavage of NS2A/2B by NS2B3. ( A) Genome structure and cleavage sites of flavivirus polyprotein. ( B) Conservative amino acid residues near the NS2A/2B cleavage site by comparison of different flaviviruses, including DENV, JEV WNV, YFV, TBEV, BGAV, ZIKA and KUN. (C) Overexpression of DTMUV NS2A/2B in transfected DEFs. DEF cells were transfected with different concentrations of pCAGGS-Myc-NS2A/2B-Flag and the cells were harvested 24 h post transfection. ( D, E) Cleavage of NS2A/2B by NS2B3. DEF cells were cotransfected with plasmids expressing NS2A/2B and with different concentrations of NS2B3 plasmids, and proteins of interest were detected by WB 24 h post transfection. ( D) Mouse anti-Flag monoclonal antibody was used as the primary antibodies, ( E) Mouse anti-Myc monoclonal antibody was used as the primary antibody. The original Article has been corrected.