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  • 标题:Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
  • 本地全文:下载
  • 作者:Samiah Shahid ; Wajeehah Shahid ; Jawaria Shaheen
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2021
  • 卷号:11
  • DOI:10.1038/s41598-021-02257-4
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Dysregulation of non-coding microRNAs during the course of tumor development, invasion and/or progression to the distant organs, makes them a promising candidate marker for the diagnosis of cancer and associated malignancies. This exploratory study aims at evaluating the usefulness of plasma concentration of circulating mir-146a as a non-invasive biomarker for acute lymphoblastic leukemia (ALL). Total RNA including miRNA was isolated from 110 plasma samples of patients ( n  = 66), healthy controls ( n  = 24) and follow up ( n  = 20) cases and reverse transcribed. Relative concentrations were assessed using real-time quantitative PCR and fold-change was calculated by 2 −ΔΔCt method. Finally, relative concentrations were correlated to clinicopathological factors. Patients ( n  = 66) were analyzed to determine fold expression of miR-146a in plasma samples of ALL. Before chemotherapy, pediatric ( n  = 42) and adult ( n  = 24) showed overexpression of miR-146a compared with healthy controls ( P  < 0.0001). There was no effect of age and gender on mir-146a expression in plasma. mirR-146a expression was independent of clinical and hematological features. Moreover, miR-146a levels in plasma of paired samples ( n  = 20) after treatment showed significant decrease in expression ( P  < 0.001). Expression of plasma miR-146a may be utilized as non-invasive marker to diagnose and predict prognosis in pediatric and adult patients with ALL. Moreover predicted targets may be utilized for ALL therapy in future.
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