首页    期刊浏览 2024年12月05日 星期四
登录注册

文章基本信息

  • 标题:The threat of persistent bacteria and fungi contamination in tuberculosis sputum cultures
  • 本地全文:下载
  • 作者:Grace Muzanyi ; Aber peace ; Bonny Wamuntu
  • 期刊名称:African Health Sciences
  • 印刷版ISSN:1680-6905
  • 电子版ISSN:1729-0503
  • 出版年度:2021
  • 卷号:21
  • 期号:2
  • 页码:628-632
  • DOI:10.4314/ahs.v21i2.18
  • 语种:English
  • 出版社:Makerere University Medical School(Uganda)
  • 摘要:Background Tuberculosis (TB) sputum culture contaminants make it difficult to obtain pure TB isolates.We aimed to study and identify persistent TB sputum culture contaminants post the standard laboratory pre-culture sample decontamination techniques. Methods This was a longitudinal study of TB sputum culture contamination for a cohort of TB patients on standard treatment at: baseline, during TB treatment and post TB treatment. Sputum samples were decontaminated with 1.5%NaOH and neutralized using 6.8 Phosphate buffer solution.Sputum was then inoculated into MGIT (mycobactrial growth indicator tube) supplemented with 0.8ml PANTA. A drop of each positive MGIT culture was sub cultured onto blood agar and incubated for 48 hours at 35 -37OC.Any growth was identified using growth characteristics and colony morphology. Results From October 2017 through May 2019;we collected 8645 sputum samples of which 8624(99.8%) were eligible and inoculated into MGIT where 2444(28.3%)samples were TB culture positive and 255(10.4%)were positive for contaminants: 237 none-tuberculosis bacteria, 12 fungi and 6 mixed(none-tuberculous bacteria+fungi). There was no statistically significant difference between none tuberculosis bacteria and fungi in the treatment (OR=1.4,95%CI:0.26–7.47,p=0.690) and the post treatment TB phases(OR=2.02,95%CI:0.38–10.79,p=0.411)Vs baseline. Conclusion None-tuberculous bacteria and fungi dominate the plethora of TB sputum culture contamination and persist beyond the standard laboratory pre-culture decontamination algorithm.
  • 关键词:enBacteria;Fungi;Inoculation;PANTA (Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim, Azlocillin)
国家哲学社会科学文献中心版权所有