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标题：Critical roles of a housekeeping sortase of probiotic Bifidobacterium bifidum in bacterium–host cell crosstalk
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作者：Eiji Ishikawa ; Tetsuya Yamada ; Kazuaki Yamaji 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：11
页码：1-22
DOI：10.1016/j.isci.2021.103363
语种：English
出版社：Elsevier
摘要：SummaryBifidobacterium bifidumYIT 10347 (BF-1) is adhesivein vitro. Here we studied the molecular aspects of the BF-1 adhesion process. We identified and characterized non-adhesive mutants and found that a class E housekeeping sortase was critical for the adhesion to mucin. These mutants were significantly less adhesive to GCIY cells than was the wild type (WT), which protected GCIY cells against acid treatment more than did a non-adhesive mutant. The non-adhesive mutants aberrantly accumulated precursors of putative sortase-dependent proteins (SDPs). Recombinant SDPs bound to mucin. Disruption of the housekeeping sortase influenced expression of SDPs and pilus components. Mutants defective in a pilin or in an SDP showed the same adhesion properties as WT. Therefore, multiple SDPs and pili seem to work cooperatively to achieve adhesion, and the housekeeping sortase is responsible for cell wall anchoring of its substrates to ensure their proper biological function.Graphical abstractDisplay OmittedHighlights•Non-adhesive mutants ofB. bifidumlack a functional housekeeping sortase•A non-adhesive mutant is less protective for cultured cells against acid stress•The housekeeping sortase is important for anchoring adhesion factors to cell wall•B. bifidumhas species-specific sortase-dependent proteinsMicrobiology; Microbiome; Evolutionary ecology