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标题：Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
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作者：Bidhayak Chakraborty ; Bidhayak Chakraborty ; Raju Suresh Kumar 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：8
页码：1-9
DOI：10.1016/j.jksus.2021.101660
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractObjectiveThe bio-synthesis of silver nanoparticles (AgNPs) is regarded as environment friendly and cost effective method compared to physical and chemical synthesis methods. Silver (Ag) is an important noble metal due to its tremendous use in research and medical fields throughout the world. This research work was aimed at green synthesis and characterizations of AgNPs fromG. glaucaleaf extract and evaluation of their bioactive potential.MethodsIn the current research work, synthesis of AgNPs fromG. glaucaaqueous leaf extracts was performed and was characterized by UV–Visible (UV–Vis.), Fourier transform infrared (FTIR), Atomic force microscopy (AFM), Scanning electron microscopy (SEM), Energy dispersive spectroscopy (EDS), High-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), Thermo gravimetric analysis (TGA) and Zeta potential exploration. Later, antioxidant, antimicrobial activity was examined against various pathogenic organisms and anticancer activities of AgNPs were carried out against SK-HEP1 cell line.ResultsThe first indication of synthesis of nanoparticles was the change in color from yellowish to brown. UV–Vis. band exhibited surface plasmon resonance (SPR) at 402 nm. FTIR analysis revealed the probable bio-molecules including alkanes, alkenes, aromatics, aromatic phosphates, imine or oxime, etc. subjected to reduction of silver ions to metallic silver. The AFM, SEM and TEM analysis reported the particles were spherical shaped, poly-dispersed, 17 to 40 nm in size. The zeta potential analysis expressed a peak at –32.0 ± 0.5 mV and suggested the particles with significant long term stability. The TGA analysis revealed the stability of AgNPs at high temperature (179 ℃). The antioxidant assay of AgNPs unveiled an effective dose dependent increase in scavenging activity. Antimicrobial activity showed efficient inhibitory activity againstP. aeruginosaandC. glabrata. Lastly, the AgNPs revealed a strong anticancer activity against SK-HEP1 liver cancer cell line with an IC50value of 19.12 μg/mL.ConclusionsTherefore, it could be concluded that a substantial in-vivo investigations are needed for antioxidant, antibacterial and anticancer activities, so that it will be useful in medical field in future.
关键词：Galphimia glauca;Green synthesis;Antioxidant;Antimicrobial;Anticancer;SK-HEP1 cell line;Silver nanoparticles