文章基本信息

标题：Chromosome-scale genome sequencing, assembly and annotation of six genomes from subfamily Leishmaniinae
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作者：Almutairi, Hatim ; Urbaniak, Michael D. ; Bates, Michelle D. 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2021
卷号：8
期号：1
页码：1-9
DOI：10.1038/s41597-021-01017-3
语种：English
出版社：Nature Publishing Group
摘要：We provide the raw and processed data produced during the genome sequencing of isolates from six species of parasites from the sub-family Leishmaniinae: Leishmania martiniquensis (Thailand), Leishmania orientalis (Thailand), Leishmania enriettii (Brazil), Leishmania sp. Ghana, Leishmania sp. Namibia and Porcisia hertigi (Panama). De novo assembly was performed using Nanopore long reads to construct chromosome backbone scaffolds. We then corrected erroneous base calling by mapping short Illumina paired-end reads onto the initial assembly. Data has been deposited at NCBI as follows: raw sequencing output in the Sequence Read Archive, finished genomes in GenBank, and ancillary data in BioSample and BioProject. Derived data such as quality scoring, SAM files, genome annotations and repeat sequence lists have been deposited in Lancaster University鈥檚 electronic data archive with DOIs provided for each item. Our coding workflow has been deposited in GitHub and Zenodo repositories. This data constitutes a resource for the comparative genomics of parasites and for further applications in general and clinical parasitology.