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标题：Thermoxylanolytic and Thermosaccharolytic Potential of a Heat Adapted Bacterial Consortium Developed From Goat Rumen Contents
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作者：Khusboo Lepcha ; Arijita Basak ; Subham Kanoo 等
期刊名称：Frontiers in Energy Research
电子版ISSN：2296-598X
出版年度：2021
卷号：9
DOI：10.3389/fenrg.2021.755779
语种：English
出版社：Frontiers Media S.A.
摘要：Thermoactive xylanases have important applications in the industrial deconstruction of lignocellulosic plant biomass, due to their sustained activity even at high temperature conditions of industrial bioreactors. We herein report the development of a thermoactive xylanolytic microbial consortium from the semi-digested contents of goat rumen and characterization of the xylanolytic enzyme cocktail produced by it. The consortium exhibited maximum endoxylanase activity at pH6 and at 60°C. Zymogram analysis revealed the production of multiple xylanases. The xylanase cocktail was stable over a pH range of 5–9 after pre-incubation for 3 h. It retained 74% activity after pre-incubation (60°C) for 50 min. It’s activity was enhanced in presence of β-mercaptoethanol, NH4+, Mg2⁺ and Ca2⁺, whereas Hg2⁺ had an inhibitory effect. The xylanolytic cocktail was further utilized for the saccharification of alkali pre-treated rice straw and mushroom spent rice straw. Saccharification was studied quantitatively using the dinitrosalicylic acid method and qualitatively using scanning electron microscopy. Results indicated the potential of the xylanolytic cocktail for the saccharification of rice straw and highlighted the significance of chemical and/or biological pre-treatment in improving the accessibility of the substrate towards the xylanase cocktail.