文章基本信息

标题：V _m-related extracellular potentials observed in red blood cells
本地全文：下载
作者：Michael Pycraft Hughes ; Emily J. Kruchek ; Andrew D. Beale 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-98102-9
语种：English
出版社：Springer Nature
摘要：Even in nonexcitable cells, the membrane potential V m is fundamental to cell function, with roles from ion channel regulation, development, to cancer metastasis. V m arises from transmembrane ion concentration gradients; standard models assume homogeneous extracellular and intracellular ion concentrations, and that V m only exists across the cell membrane and has no significance beyond it. Using red blood cells, we show that this is incorrect, or at least incomplete; V m is detectable beyond the cell surface, and modulating V m produces quantifiable and consistent changes in extracellular potential. Evidence strongly suggests this is due to capacitive coupling between V m and the electrical double layer, rather than molecular transporters. We show that modulating V m changes the extracellular ion composition, mimicking the behaviour if voltage-gated ion channels in non-excitable channels. We also observed V m -synchronised circadian rhythms in extracellular potential, with significant implications for cell–cell interactions and cardiovascular disease.