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标题：Proteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet
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作者：Belinda Yau ; Sheyda Naghiloo ; Alexis Diaz-Vegas 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：10
页码：1-21
DOI：10.1016/j.isci.2021.103099
语种：English
出版社：Elsevier
摘要：SummaryPancreatic islets are essential for maintaining physiological blood glucose levels, and declining islet function is a hallmark of type 2 diabetes. We employ mass spectrometry-based proteomics to systematically analyze islets from 9 genetic or diet-induced mouse models representing a broad cross-section of metabolic health. Quantifying the islet proteome to a depth of >11,500 proteins, this study represents the most detailed analysis of mouse islet proteins to date. Our data highlight that the majority of islet proteins are expressed in all strains and diets, but more than half of the proteins vary in expression levels, principally due to genetics. Associating these varied protein expression levels on an individual animal basis with individual phenotypic measures reveals islet mitochondrial function as a major positive indicator of metabolic health regardless of strain. This compendium of strain-specific and dietary changes to mouse islet proteomes represents a comprehensive resource for basic and translational islet cell biology.Graphical abstractDisplay OmittedHighlights•Most comprehensive mouse islet proteome library generated to date•Quantification of islet proteomic changes across 6 strains of mice on 2 diets•Islet mitochondrial function revealed as strain-independent regulator of metabolic healthAnimal physiology; Diabetology; Proteomics.