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标题：Author Correction: 58-F, a flavanone from Ophiopogon japonicus, prevents hepatocyte death by decreasing lysosomal membrane permeability
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作者：Xiaofeng Yan ; Tingjie Ye ; Xudong Hu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-98198-z
语种：English
出版社：Springer Nature
摘要：Correction to: Scientific Reports 10.1038/srep27875, published online 16 June 2016 This Article contains an error in Figure 8, where the treatments below the western blots in the panels E, F and G do not match the western blots. The correct Figure 8 and accompanying legend appear below. Figure 8 Effect of NAC on ROS content, cell death, lysosomal membrane permeability and the leakage of CatB to the cytosol. To identify the effect of NAC in vitro, cells were treated with 100 μg/ml of NAC for 12 h followed by an additional 2 h with 500 μM H 2O 2 for the assay with FACS. For the ROS content assay, the cells were treated with 100 μg/ml NAC for 24 h followed by 500 μM H 2O 2 for different amounts of time. In other assays, the cells were treated with 100 μg/ml NAC for 16 h followed by an additional 8 h with 500 μM H 2O 2. ( A) The Annexin V/PI assay with FACS is shown. ( B) The ROS contents in cells is shown. ( C) LysoTracker Green staining (scale bar = 10 μm) is shown. ( D) Quantification of the LysoTracker Green staining is shown. ( E, H) Levels of LAMP1 protein were measured in cells by Western blotting and quantification is shown. ( F, G, I, J) Cat B/D levels were measured in the cytosol/whole lysate by Western blotting and the quantification is shown (*p < 0.05, **p < 0.01).