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标题：Cerebral excitability in pup rats prenatally exposed to 1-bromopropane is suppressed by bromide accumulated in the brain
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作者：Yukiko FUETA ; Mitsuo HINOUE ; Hazuki NOGUCHI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：2021
卷号：59
期号：4
页码：239-248
DOI：10.2486/indhealth.2020-0098
语种：English
出版社：National Institute of Occupational Safety and Health
摘要：Previously, we reported that prenatal exposure to 1-bromopropane (1-BP) causes the accumulation of bromide (Br -) in the brain of rat pups. Here, we aimed to investigate the effects of Br - accumulation in rat pups prenatally exposed to 1-BP vapor. Dam rats were exposed to 1-BP (400 or 700 ppm; 1-BP group) by inhalation, or to NaBr (20 mM; Br - group) in drinking water during gestation days 1–20. We also analyzed pentylenetetrazole (PTZ, 60 mg/kg, ip)-induced behavioral changes in pups prenatally exposed to 1-BP or Br - on postnatal day (PND) 14. PTZ-induced epileptic convulsions were inhibited in both 1-BP (700 ppm) and Br - groups. The inhibition of neuronal excitability induced by Br - was evaluated electrophysiologically using the hippocampal slices obtained from PND14–16 pups. PTZ (2 mM) failed to induce epileptiform discharge in the presence of 1.2 mM Br - in the slices obtained from the control group. However, it induced epileptiform discharge following the removal of Br -, by perfusing artificial cerebrospinal fluid into the slices obtained from the Br - group. Our results indicate that Br - accumulates in the brain of neonatal rat pups prenatally exposed to 1-BP vapor suppressed neuronal excitability.
关键词：enPrenatal exposure;1-bromopropane;Bromide;Neurotoxicity;Pentylenetetrazole;Hippocampal slice