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标题：Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples
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作者：Yumei Liu ; Hao Yang ; Rui Wan 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2021
卷号：11
期号：8
DOI：10.3390/bios11080274
语种：English
出版社：MDPI Publishing
摘要：Lead (Pb 2+) pollution is a serious food safety issue, rapid detection of Pb 2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb 2+ supervision in food samples. Pb 2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb 2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb 2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb 2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb 2+ testing associated with food safety.
关键词：enG-quadruplex;nucleic acid probes;lead pollution;food safety;homogeneous detection