文章基本信息

标题：Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR ₄₆ azole resistance gene
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作者：Plinio Trabasso ; Tetsuhiro Matsuzawa ; Teppei Arai 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-96651-7
语种：English
出版社：Springer Nature
摘要：Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets . It also differentiated strains with TR 46 tandem repeats from those with TR 34 tandem repeats. These results showed this TR 46-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR 46 tandem repeats.