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标题：In situ observation of mitochondrial biogenesis as the early event of apoptosis
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作者：Chang-Sheng Shao ; Xiu-Hong Zhou ; Yu-Hui Miao 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：9
页码：1-35
DOI：10.1016/j.isci.2021.103038
语种：English
出版社：Elsevier
摘要：SummaryMitochondrial biogenesis is a cell response to external stimuli which is generally believed to suppress apoptosis. However, during the process of apoptosis, whether mitochondrial biogenesis occurs in the early stage of the apoptotic cells remains unclear. To address this question, we constructed the COX8-EGFP-ACTIN-mCherry HeLa cells with recombinant fluorescent proteins respectively tagged on the nucleus and mitochondria and monitored the mitochondrial changes in the living cells exposed to gamma-ray radiation. Besidesin situdetection of mitochondrial fluorescence changes, we also examined the cell viability, nuclear DNA damage, reactive oxygen species (ROS), mitochondrial superoxide, citrate synthase activity, ATP, cytoplasmic and mitochondrial calcium, mitochondrial mass, mitochondrial morphology, and protein expression related to mitochondrial biogenesis, as well as the apoptosis biomarkers. As a result, we confirmed that significant mitochondrial biogenesis took place preceding the radiation-induced apoptosis, and it was closely correlated with the apoptotic cells at late stage. The involved mechanism was also discussed.Graphical abstractDisplay OmittedHighlights•Dual fluorescence approach was used forin situobservation of living cell processes•Radiation-induced effects of mitochondrial biogenesis and apoptosis were observed•Relationship between mitochondrial biogenesis and apoptosis was revisited•Assessing early mitochondrial biogenesis is critical for predicting later fate of cellsBiochemistry methods; Biomolecular engineering; Cell biology