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标题：Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2
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作者：Jun-Gyu Park ; Fatai S. Oladunni ; Mohammed A. Rohaim 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：9
页码：1-27
DOI：10.1016/j.isci.2021.102941
语种：English
出版社：Elsevier
摘要：SummaryGlobal deployment of an effective and safe vaccine is necessary to curtail the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety, and protective efficacy against SARS-CoV-2. Intranasal administration of recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 to mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T-cell-mediated immunity. Hamsters immunized with two doses of vaccine showed complete protection from lung infection, inflammation, and pathological lesions following SARS-CoV-2 challenge. Importantly, administration of two doses of intranasal rNDV-S vaccine significantly reduced the SARS-CoV-2 shedding in nasal turbinate and lungs in hamsters. Collectively, intranasal vaccination has the potential to control infection at the site of inoculation, which should prevent both clinical disease and virus transmission to halt the spread of the COVID-19 pandemic.Graphical abstractDisplay OmittedHighlights•Vaccine induces high levels of neutralizing Abs and T-cell-mediated immunity•Vaccine ameliorates lung inflammation and pathology in hamster induced by SARS-CoV-2•The SARS-CoV-2 remains undetectable in lungs and nasal turbinates of vaccinated hamster•Two doses of intranasal vaccine show complete protection against SARS-CoV-2 challengeImmunology; Virology.