文章基本信息

标题：Cell instructive Liquid Crystalline Networks for myotube formation
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作者：Daniele Martella ; Michele Mannelli ; Roberta Squecco 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：9
页码：1-15
DOI：10.1016/j.isci.2021.103077
语种：English
出版社：Elsevier
摘要：SummaryDevelopment of biological tissuesin vitrois not a trivial task and requires the correct maturation of the selected cell line. To this aim, many attempts were done mainly by mimicking the biological environment using micro/nanopatterned or stimulated scaffolds. However, the obtainment of functional tissuesin vitrois still far from being achieved. In contrast with the standard methods, we here present an easy approach for the maturation of myotubes toward the reproduction of muscular tissue. By using liquid crystalline networks with different stiffness and molecular alignment, we demonstrate how the material itself can give favorable interactions with myoblasts helping a correct differentiation. Electrophysiological studies demonstrate that myotubes obtained on these polymers have more adult-like morphology and better functional features with respect to those cultured on standard supports. The study opens to a platform for the differentiation of other cell lines in a simple and scalable way.Graphical abstractDisplay OmittedHighlights•Liquid crystalline network are able to induce myogenesis of C2C12 myoblasts•Myogenesis is obtained without surface modifications or external stimulation•Mature myotube physiological features are obtained on liquid crystalline networksCell; Tissue Engineering; Biomaterials