摘要:<i>Aureobasidium pullulans</i> is a yeast-like fungus found on the surface of the grape berries that has been proven to act as a biocontrol agent for the management of grey mould disease caused by <i>Botrytis cinerea</i>. In this work, an indigenous strain of <i>A. pullulans</i> isolated from grape berries and selected according to the in vitro activity against <i>B. cinerea</i>, was used in vineyards of the winery where it originated, in comparison with a commercial product containing two <i>A. pullulans</i> strains with the aim of assessing its effectiveness as a biocontrol agent. The experimental design included daily meteorological data registration and the early defoliation of grapevines as treatments. The monitoring of <i>A. pullulans</i> strains on grape berries by plate counts and molecular methods as well as of <i>B. cinerea</i> symptoms on grape bunches was performed in the different trials from the end of flowering to the harvest time. Results highlighted that although no significant differences (<i>p</i> < 0.05) in the occurrence of <i>B. cinerea</i> were detected according to different treatments, the mean incidence of symptomatic berries ranged from 7 to 16%, with the lowest values recorded in bunches treated with the indigenous <i>A. pullulans</i> strain. The efficacy of the biocontrol agent was affected more by meteorological conditions than the defoliation practice.