文章基本信息

标题：Enriched Synthesis of Magnetosomes by Expanding the Magnetospirillum magneticum AMB-1 Culture at Optimal Iron Concentration
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作者：Yazhen Hong ; Ranjith Kumar Kankala ; Ruqi Yu 等
期刊名称：Maine Policy Review
印刷版ISSN：2312-7481
出版年度：2021
卷号：7
期号：8
页码：115
DOI：10.3390/magnetochemistry7080115
语种：English
出版社：The University of Maine
摘要：The Magnetospirillum magneticum AMB-1 species is one of the most widely used magnetotactic bacterial strains for producing magnetosomes under laboratory conditions. Nevertheless, there exist several challenges in expanding and purifying the AMB-1 culture due to the restricted culture conditions. In an attempt to enrich the production of magnetosomes, this study reports the utilization of fermenter culture, which substantially promotes the cell densities at different concentrations of iron content. The experimental results confirmed magnetosomes’ high yield (production rate of 21.1 mg L−1) at the iron content of 0.2 μmol L−1. Moreover, different characterization techniques systematically confirmed the coated lipid membrane, particle size, dispersity, stability, and elemental composition of magnetosomes. Notably, the fermenter culture-based process resulted in highly discrete, dispersed, and stable magnetosomes with an average particle diameter of 50 nm and presented the integrated lipid membrane around the surface. The chemical composition by EDS of magnetosomes represented the presence of various elements, i.e., C, O, Na, P, and Fe, at appropriate proportions. In conclusion, the culture method in our study effectively provides a promising approach towards the culture of the magnetotactic bacterium for the enriched production of magnetosomes.