Waste resulting from industrial poultry production systems is becoming an increasingly significant environmental problem in the US, threatening both soil and water quality. The goal of this study was to assess the spatial variability and interactions of selected soil properties (physical, chemical, and biochemical), viz., particle size, pH, enzymatic activity, Soil Organic Carbon (SOC), and Total Nitrogen (TN), across an agricultural landscape used for industrial poultry production. The measured soil properties were separated according to biochemical constituents and soil texture based on the first two principal components, accounting for approximately 60% of the variability across the site. These principal components were then used to generate soil surface maps, indicating areas of possible catalytic activity. Surface maps showed possible increases in biochemical activity around areas of stored poultry litter, suggesting the utility of these methods in determining changes to soil management.