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标题：Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode
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作者：Ahmed A. Heflish ; Amira E. Hanfy ; Mohammad Javed Ansari 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：6
页码：1-8
DOI：10.1016/j.jksus.2021.101516
语种：English
出版社：Elsevier
摘要：AbstractNanoparticles nowadays are an effective control agent against most phytopathogens. However, there are many reports on their antimicrobial activity and little as a nematicide. In this study, green biosynthesis of silver nanoparticles (Ag NPs) usingAcalypha wilkesianaaqueous leaf extract was achieved. X-ray diffraction patterns confirmed the crystalline structure with face-centred cubic (fcc), where is the size was nearly 20 nm. SEM images of the Ag NPs show the spherical shape and in the range from 10 to 30 nm. Various functional groups, capping, and stability agents were identified using Fourier transmission infrared spectroscopy (FTIR). The nematicidal activity of biosynthesized Ag NPs conc. 25, 50, and 100 µg/mL were evaluatedin vitroagainst root-knot nematode (Meloidogyne incognita), egg hatching (6 days after) and movement after 24 and 48 h. The Ag NPs (100 µg/ml) application after 48 h was the most effective treatment that showed 53.3% of nematode mortality. Overall, the efficiency of bio-Ag NPs reduced the nematode activity, mortality, egg hatching, and movement of larvae. To our knowledge, this is the first report of nematicidal action of biosynthesized Ag NPs usingA. wilkesianaaqueous extract, and it could be recommended to manage the plant-parasitic nematode as it is simple, stable, cost-effective and keep the environment safe.
关键词：Ag NPs;Green biosynthesis;Acalypha wilkesiana;FTIR;SEM;Nematicidal activity