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标题：Anti-bacterial effect of marine sea grasses mediated endophytic actinomycetes against K. pneumoniae
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作者：Khalid S. Almaary ; Naiyf S. Alharbi ; Shine Kadaikunnan 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：6
页码：1-7
DOI：10.1016/j.jksus.2021.101528
语种：English
出版社：Elsevier
摘要：AbstractIn this study, the pure, pale white, powdery nature and pigment produced endophytic actinomycetes strains were isolated from marine Sea grasses sample. The isolated strains were endophytes as confirmed by surface sterilization and validation methods. The anti-bacterial efficacy materials of the crude compound were extracted by liquid–liquid extraction using various organic solvents. Anti-bacterial efficacy of the isolated actinomycetes strain RMN 10 was exhibited excellent anti-bacterial activity againstK. pneumoniaeat increasing concentration. In addition, the minimum inhibition concentration (MIC) of the crude actinomycetes compound has excellent anti-bacterial activity at 250 µg/mL concentration and this concentration was fixed as a MIC. Further, the structural and shape damages of actinomycetes crude compound treatedK. pneumoniaecells were clearly identified in SEM images. All the invitro experiments were proved that the actinomycetes compound has excellent anti-bacterial activity againstK. pneumoniae.
关键词：Marine environment;Actinomycetes;Bacterial inactivation;Minimum inhibition concentration;Scanning electron microscope