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标题：In silico screening and validation of KPHS_00890 protein of Klebsiella pneumoniae proteome: An application to bacterial resistance and pathogenesis
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作者：Pavan Heggadadevanakote Kendaganna ; Chandan Shivamallu ; Govindaraju Shruthi 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：6
页码：1-10
DOI：10.1016/j.jksus.2021.101537
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractIntroductionNosocomial infections are a notorious subset of infectious diseases, varying between 10% and 20% prevalence worldwide. The infections are concomitant with various treatment complications, multiple-drug resistance, and a high degree of virulence.Klebsiella pneumoniaeis a gram-negative bacteria of nosocomial importance.ObjectivesOur current study is gauged to reason and understand why, despite treatment with cutting-edge medicines and technology, theK. pneumoniaeremains elusive.MethodsUsing variousin silicotools, the KPHS_00890 hypothetical protein ofK. pneumoniaesubsp. pneumoniae HS11286 was identified and annotated.ResultsA thorough investigation revealed that KPHS_00890 hypothetical protein is a bifunctional 5′-nucleotidase, an enzyme catalyzing the degradation of nucleotides to nucleosides.ConclusionsScrutiny and review of the 5′-nucleotidase function across various species ascertained its pertinent role in immune evasion, by suppressing inflammatory responses. Thus, having identified the KPHS_00890 hypothetical protein ofK. pneumoniaesubsp. pneumoniae HS11286 as a 5′-nucleotidase, we propose that it may be involved in an immune evasion strategy during infection pathogenesis.
关键词：Molecular docking;Homology modeling;Phylogenetic tree;5′-nucleotidase;Klebsiella pneumoniae;Multiple sequence alignment