文章基本信息

标题：Two base pair deletion in IL2 receptor γ gene in NOD/SCID mice induces a highly severe immunodeficiency
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作者：Inseon Bak ; Doo-Jin Kim ; Hyoung-Chin Kim 等
期刊名称：Laboratory Animal Research
印刷版ISSN：1738-6055
电子版ISSN：2233-7660
出版年度：2020
卷号：36
期号：1
页码：1-11
DOI：10.1186/s42826-020-00048-y
语种：English
出版社：BioMed Central Ltd.
摘要：Genome editing has recently emerged as a powerful tool for generating mutant mice. Small deletions of nucleotides in the target genes are frequently found in CRISPR/Cas9 mediated mutant mice. However, there are very few reports analyzing the phenotypes in small deleted mutant mice generated by CRISPR/Cas9. In this study, we generated a mutant by microinjecting sgRNAs targeting the IL2 receptor γ gene and Cas9 protein, into the cytoplasm of IVF-derived NOD.CB17/Prkdcscid/JKrb (NOD/SCID) mice embryos, and further investigated whether a 2 bp deletion of the IL2 receptor γ gene affects severe deficiency of immune cells as seen in NOD/LtSz-scid IL2 receptor γ−/− (NSG) mice. Our results show that the thymus weight of mutant mice is significantly less than that of NOD/SCID mice, whereas the spleen weight was marginally less. T and B cells in the mutant mice were severely deficient, and NK cells were almost absent. In addition, tumor growth was exceedingly increased in the mutant mice transplanted with HepG2, Raji and A549 cells, but not in nude and NOD/SCID mice. These results suggest that the NOD/SCID mice with deletion of 2 bp in the IL2 receptor γ gene shows same phenotype as NSG mice. Taken together, our data indicates that small deletions by genome editing is sufficient to generate null mutant mice.
关键词：CRISPR/Cas9;IL2 receptor ;Small deletions;Immunodeficient mouse