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标题：Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging
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作者：Ehn-Kyoung Choi ; Haiyu Guo ; Jae-Kwon Choi 等
期刊名称：Laboratory Animal Research
印刷版ISSN：1738-6055
电子版ISSN：2233-7660
出版年度：2015
卷号：31
期号：3
页码：148-152
DOI：10.5625/lar.2015.31.3.148
语种：English
出版社：BioMed Central Ltd.
摘要：In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 µg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 µg/mL. High concentrations (≥250 µg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 µg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 µM, reaching 80% inhibition at 125 µM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.
关键词：Rosa hybrida;white rose petal extract;matrix metalloproteinase-1;elastase;tyrosinase